EcoRI-HF ®. High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer ( CutSmart ® Buffer ). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X) .
EcoRI has a High Fidelity version EcoRI-HF ® . High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
13 rows · EcoRI Monarch® Plasmid Miniprep Kit Monarch® DNA Gel Extraction Kit Monarch® PCR.
EcoRI – HF ® has the same specificity as EcoRI (NEB #R0101), but it has been engineered for reduced star activity. Blocked by some combinations of overlapping CpG methylation. Contents ©New England Biolabs Ltd. New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc. New England Biolabs, Inc. is an ISO …
Whether you are setting up digests for 5-15 minutes or overnight, or using varying amounts of enzymes, HF enzymes ensure the performance you need. EcoRI-HF® ( NEB #R3101 ) shows no star activity in overnight digests, even when used at higher concentrations. 50 ?l reactions were set up using 1 ?g of Lambda DNA, the indicated amount of enzyme and the recommended reaction buffer.
High-Fidelity (HF®) Restriction Endonucleases | NEB, EcoRI-HF® | NEB, EcoRI – Wikipedia, EcoRI | NEB, EcoRI – HF ® (New England Biolabs, Cat. #R3101). Any restriction enzyme may be used, provided that it cuts the plasmid to leave 5? overhangs that can be filled in with a suitable radiolabeled dNTP • Molecular biology grade agarose • Horizontal gel electrophoresis rig, casting tray (~ 10 cm × 10 cm), and comb •, Thermo Scientific EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA …
EcoRI (pronounced eco R one) is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.The Eco part of the enzyme’s name originates from the species from which it was isolated – E denotes generic name which is Escherichia and co …
HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
HindIII, Restriction Enzyme, BamHI, Polymerase chain reaction, EcoRV
